Leishmania donovani

Bone marrow biopsy
Macrophage with amastigotes (3 - 4 x 2 μm)

• The amastigote forms multiply in the host cell until the cell is destroyed and the liberated cell are phagocytized by other reticuloendothelial cells (Macrophage)

Leishmania donovani
Spleen
Macrophage with amastigotes (3 - 4 x 2 μm)

The amastigote forms multiply in the host cell until the cell is destroyed and the liberated cell are phagocytized by other reticuloendothelial cells (Macrophage).

Tongue biopsy
Macrophage with amastigotes (3 - 4 x 2 μm)

• The amastigote forms multiply in the host cell until the cell is destroyed and the liberated cell are phagocytized by other reticuloendothelial cells (Macrophage) or in this case muscle cells of the tongue (HIV positiv patient)

Culture; promastigote form
Length: 14 - 20 μm

• Giemsa stained culture material
• In culture the organism transforms in the vector transmitted form
• the promastigote form
• The kinetoplast in this stage is in the anterior end and there is a free flagellum