Introduction to Diagnostic Medical Parasitology
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Key Diagnostic Features
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Exercises
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Exercises for plasmodia
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Which shape does the parasite have?
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Ring form with 1 nucleus or 2
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What is the aspect of the ring form (young trophozoite) and its host cell?
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Ring form with 1 or 2 nuclei, ring fragile,sometimes accollé; often multiple rings per cell
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Ring thick, parasitized erythrocyte normal size with Maurer’s clefts
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Ring thick, medium size, cytoplasm compact, no Schüffner’s dots or Maurer’s clefts. Parasitized erythrocyte normal size or even smaller
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Ring thick and big, no Schüffner’s dots or Maurer’s clefts. Parasitized erythrocyte enlarged
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Ring thick and big with Schüffner’s dots. Parasitized erythrocyte enlarged, oval
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Parasite with 1 nucleus, irregularily shaped or almost as big as erythrocyte (amoeboid trophozoite or gametocyte with compact cytoplasm)
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Are vacuoles visible in the parasite?
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The parasite has no vacuoles (gametocyte)
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What is the shape of the parasite?
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Gametocyte is
(Macrogametocyte (left): blue cytoplasm, elongated, chromatin mass concentrated in the middle Microgametocyte(right): more stumpy, cytoplasm more redish, chromatin mass diffuse) -
Gametocyte is round or oval
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Which changes of the parasitized erythrocyte can be observed?
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Parasitized erythrocyte is larger than normal erythrocyte, round, Schüffner’s dots present
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Parasitized erythrocyte enlarged, oval with fimbriated edges and pronounced Schüffner’s dots
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Parasitized erythrocyte not enlarged or even smaller, no Schüffner’s dots or Maurer`s clefts
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The parasite contains one or several small vacuoles (mature trophozoite)
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What is the shape of the parasite and its host cell?
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Parasite has an amoeboid form; parasitized erythrocyte enlarged with Schüffner’s dots
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Parasite has an amoeboid form; parasitized erythrocyte enlarged, oval with fimbriated edges and pronounced Schüffner’s dots
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Parasitized has a band form; erythrocyte not enlarged or even smaller, no Schüffner’s dots or Maurer`s clefts
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Parasite with more than 1 nucleus, almost as big as erythrocyte (schizont)
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How many nuclei (merozoites) are visible within the schizont?
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More than 12 (up to 24) merozoites
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Less than 12 merozoites
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What are the characteristic signs of the parasitized erythrocyte?
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Erythrocyte enlarged (round), with Schüffner’s dots; Merozoites not clearly separated (immature schizont)
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Erythrocyte enlarged (oval with fimbriated edges), with Schüffner’s dots; Merozoites not clearly separated (immature schizont) or clearly separated (mature schizont)
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Erythrocyte not enlarged or even smaller, no Schüffner’s dots or Maurer’s clefts, merozoites arranged in rosettes with central brown-yellow pigment
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Parasite is banana-shaped (Macrogametocyte (left): blue cytoplasm, elongated, chromatin mass concentrated in the middle Microgametocyte(right): more stumpy, cytoplasm more redish, chromatin mass diffuse)
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